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A: Differences in Windows 10 build version (e.g., 1809 vs 22H2) or USB controller chipsets (Intel vs ASMedia). Use the working PC as your dedicated programming machine. Usb Download Cable Gev189 Driver Windows 10
A: Physically yes, if you match voltage levels (3.3V or 5V). But the cable is overpriced for that purpose; a $5 CP2102 module is better. Need further assistance